A rapid method for purification of synthetic oligoribonucleotides
Summary
Type: HPLC Application #: 107330
Detection: UV, 260 nm Conditions: equilibrated with 20 mM NaAc, 20% acetonitrile (pH 6.5) and eluted with a linear gradient of 0 - 1.5 M KCl within 50 min at a flow rate of 2 ml/min Authors: Webster KR Source: BioTechniques 11 (1991) 658 - 661
Details
Legend: Fig. 2: Anion exchange HPLC purification of full-length oligoribonucleotides.
Products of a deprotection reaction were at first separated using a silica gel
column with the following characteristics